Twenty eight actinomycetes strains were isolated from cotton plant rhizosphere soil of North Indian region of India. The isolates were identified as actinomycetes by morphological studies. Among them 11 strain are selected on basis of cellulolytic activities by primary screening. Cellulolytic activities were exhibited by strain A1 A2 A3 A8 A11 A13 A16 A18 Grey 72 AB1 AB4 and it was confirmed by formation of clear zone of hydrolysis around the colonies. Among the isolates maximum cellulolytic activity were exhibited by strains AB4 and it was determined by Enzyme assay.
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