Adaptive changes in white dead nettle leaves during micropropagation

The white dead nettle (Lamium album L.) is a herb that was successfully subjected to in vitro micropropagation followed by ex vitro adaptation back to the natural habitat. The L. album micropropagation system offers a combination of factors (light intensity temperature carbon dioxide level humidity) that are limiting for plant growth and bioactive capacity. Here we performed comparative investigation on the primary and secondary metabolism in fully expanded L. album leaves during the consecutive growth in in vivo in vitro and ex vitro conditions. The micropropagation did not affect the general leaf anatomy but it caused permanent decrease in the number of palisade mesophyll layers and the hydrocarbon content in the essential oils. During the in vitro conditions the thylakoid membrane structure was altered the photosynthetic performance was reduced and the content of primary and secondary metabolites declined. The plant transfer back to the field led to resumption of both normal growth and general bioactive capacity. The present work broadens the understanding about the nature of plant responses towards environmental conditions.