The present book is initiated to compare the ability of severaldetection methods i.e. a serial dilution anaerobic culture and/ormicroscopic procedure a DNA probe procedure and immunologicalreagents using both an enzyme-linked immunosorbent assay (ELISA)and an indirect immunofluorescence assay (IFA) to detect a selectedpanel of periodontopathic organisms in single subgingival plaque samplestaken from periodontally diseased sites.