Antiviral Resistance in Plants

<p>Studies related to pathogen-mediated virus resistance in plants were instrumental in providing some of the historical observations which ultimately led to the vital discovery of double-stranded RNA (dsRNA)-induced gene silencing or RNA interference (RNAi) which has since revolutionized research on plant-virus interactions. In <i>Antiviral Resistance in Plants: Methods and Protocols</i> expert researchers in the field detail many of the methods which are now commonly used to study the phenomenon of RNA silencing in relation to viral infections of plants.�� These include methods and techniques for the isolation and quantitative/qualitative analyses of plant small 21-24 nucleotide RNAs such as small interfering RNAs (siRNAs) and microRNAs (miRNAs) as well as the analysis and manipulation of virus-induced gene silencing (VIGS) in both monocotyledonous and dicotyledenous plants and the use of hairpin RNA (hpRNA) transgenes.�� Written in the highly successful <i>Methods in Molecular Biology���</i> series format chapters include introductions to their respective topics lists of the necessary materials and reagents step-by-step readily reproducible laboratory protocols and key tips on troubleshooting and avoiding known pitfalls.</p><p>��</p>Authoritative and practical <i>Antiviral Resistance in Plants: Methods and Protocols</i> seeks to aid scientists in the further study of this crucially important botanical trait.