BENCH GUIDE FOR PROTEIN EXPRESSION & PURIFICATION

The guide is a self-contained volume covering all the important procedures and background knowledge related to production purification and analysis workflows related to His-tagged recombinant protein at research scale with greatly simplified user friendly and best use protocols. The manual also describes how to design a protein purification procedure advice on how to choose the ideal protein expression purification and detection systems and how to avoid pitfalls in all operations starting from protein production to final analysis of the purified protein. The manual is educational and greatly useful to beginners in the field where they can get a quick overview when entering the field; experienced researchers can also find valuable information.