Biotechnological potential of Citrus X SP essential oil (Lemon Tanja)

This study aimed to evaluate the chemical profile determine phenolic compounds (TPC) total flavonoids (TFC) and a refraction index of Citrus x sp essential oil (EO) as well as antioxidant anti-inflammatory and antiarthritic activity. The EO of Citrus x sp peels was extracted using the hydrodistillation technique and nanoemulsions were formulated using the phase inversion method. By chromatography it was possible to identify limonene as the majority component (70.25%). The TPC and TFC quantified 227.645 mg ETA g-1 and 86.57 mg EQ g-1 respectively and the refraction index quantified 1.475. For antioxidant capacity nanoemulsions present EC50 values of 9.10 10.18 and 11.28 mg L-1 for NEO3 NEO1 and NEO2. In the anti-inflammatory activity synergies S4 S5 and S7 were quantified at 4.63 11.12 and 11.03 mg L-1. For antiarthritic it is noted that S1 S6 and S8 showed excellent antiarthritic activity among the nine synergies formulated with values for EC50 of 1.9 1.69 and 1.98 mg L-1. It can be affirmed that the nanoemulsions and synergies from the EO of Citrus x sp presented satisfactory results evidencing the efficacy of the synergistic properties that these present.