Biotechnology Proteins to PCR
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ism (i. e. Saccharomyces carlsbergensis or brewers yeast) and one of its corresponding enzymes. The experiments on this organism and enzyme are not limited to the materials suggested and can be easily adapted to the desired technical level and available budget. Similarly the subse­ quent cloning experiments suggest that use of particular vectors and strains but as indicated alternative materials can be used to success­ fully perform the laboratory exercises. We would like to thank the corporate sponsors of the Biotechnology Training Institute for providing the materials and expertise for the devel­ opment of our programs and thus for the materials in this manual. These sponsors include: • Barnsteadffhermolyne Dubuque IA • Beckman Instruments Somerset NJ • Bio-Rad Laboratories Hercules CA • Boehringer Mannheim Corporation Indianapolis IN • Coming Costar Corporation Cambridge MA • FMC BioProducts Rockland ME • Kodak Laboratory Products New Haven CT • Labconco Kansas City MO • MJ Research Cambridge MA • Olympus Instruments Lake Success NY • Pharmacia Biotech Piscataway NJ • Savant Inc. Farmingdale NY • VWR Scientific Philadelphia P A We would also like to thank the following individuals for their input comments and suggestions: Tom Slyker Bernie Janoson Steven Piccoli John FordJeffGarelik Yanan Tian and Douglas Beecher. Special thanks to Alan Williams for his critique of the chromatography experiments and Shannon Gentile for her work in the laboratory. We would especial­ ly like to thank Maryann Burden for her comments and encouragement.
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