Comparative Genomics of Salmonella spp using PFGE

Salmonella spp. reported to be one of the most important food pathogen studied worldwide. Salmonella causes typhoidal and non-typhoidal diseases in humans and animals. The genetic relatedness of the isolates was correlated using bioinformatics software Applied Math. A standard salmonella isolation method was used in the bacterial isolation. Colonies from microbial plates were confirmed using MicroSEQ Salmonella spp. Detection Kits and Step One Plus RT PCR Machine from Applied Biosystems. A genetic dendrogram was generated using Applied Math software from the PFGE gels generated by Xbal digestion of the strains and using Salmonella braenderup (H9812) as reference strain. The technique was helpful in correlating the bacterial strains from poultry and environmental samples.