CRISPR-Cas system based diagnosis of infectious diseases

Since its discovery as a part of the bacterial defense mechanism the Nobel Prize-winning technology CRISPR-Cas system has revolutionized the fields of genome editing and genetic engineering. Beyond gene-editing ability scientists have leveraged its potential in the diagnosis of infectious diseases including COVID-19.This book provides a detailed understanding of CRISPR-based rapid and point-of-care diagnostic kits like SHERLOCK DETECTR FELUDA AIOD CRISPR-Cas12a etc. for the diagnosis of infectious diseases including COVID-19; the importance of isothermal nucleic acid amplification processes like RPA and LAMP; and how the results of the CRISPR-based tests can be interpreted using paper-based lateral flow strips.Because of the ease of performing the test and simple interpretation of the test results CRISPR-based tests can be used at airports ports clinics schools etc. for better disease diagnosis monitoring management and containment of infectious diseases like COVID-19.Additionally this book discusses Monoclonal Antibodies which have revolutionized the treatment for cancer arthritis autoimmune diseases etc. This book also talks about various strategies to isolate monoclonal antibodies from the COVID-19 recovered people and different ways to engineer these antibodies using hybridoma technology.