CRISPR-Cas9 ? ????????????? ????????
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???? ???????????? - ??????????? ?????? ? ???????????? ??????????? ????????????? ???????? ? ??????? ??????????? ??????? ?????????? ??? ????????? ??????????? ????????-??????????????? ?????????? ????? ? ???????????? ???????. ????????????? ????????????? ???????? ??????????? ?? ?????? ??????????????? ????????? ? ???? 16S ????. ??? ?????????????????? ?????????????????? ????? ????????? ???????? (E) ?????? 0 ? ????? 99 % ?????????????????? ???????? ????? ????? ????? ??????? ????? ????? ?? ???????? ???????????? BLASTN ? ???????????????? ??? Methylorubrum extorquens ? Pseudomonas balearica.? M. extorquens ? P. balearica ???? ???????????? ??????? CRISPR-Cas9 ???????????? ????????? ? ?????????? qPCR ???????? ??? ?????????? ???? MDH ??????????? ? 6 ??? ? CRISPR-MDH-???????????? M. extorquens. ? P. balearica ???????????? CRISPR ?????????? ???? pMMO ???????? ? 25 ???. ?????????? CRISPR- Cas9 ???? ??????? ??? ???????? ? ????????????? ? ????? ????????? ????????????? ????????????? ?????????????. ????????? ??? ???????? ? ?????? ???????????? ????????????? ???????? ??????? ???????? ? ????? ? ????? ???? ? ???? ??????? ???????????? ???????CRISPR-Cas9 ??? ????????? ????????????? ???? ???????? ? ????????????? ?????????????.
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