Demonstration of collagen fibers using Geimsa stain

Giemsa is a metachromatic stain in that many tissues/organisms stain differently than the dye color itself. The dye mixture – methylene blue azure and eosin compounds – is blue but positive staining is designated by a purplish hue. Giemsa is frequently used to identify mast cells; their granules stain positively. Of note the immunohistochemical stains “mast cell tryptase” and “CD 117” are also used to detect mast cells. Urticaria and urticaria pigmentosa are among the disease entities marked by a significant increase in mast cell volume thus Giemsa finds its utility here. Further contributing to the value of Giemsa is the positive staining of several infectious organisms; spirochetes protozoans and cutaneous Leishmaniain particular. Probably the first use of a staining solution containing methylene blue (a cationic thiazine dye) and eosin (a red anionic xanthene dye) was in 1888 by C. Chenzinsky to stain malaria parasites in blood films. F. Plehn in 1890 and E. Malachowski and D. L. Romanowsky in 1891 all independently described similar staining solutions that imparted a variety of colors to erythrocytes leukocytes and malaria parasites.