Detection Molecular of E.coli O157

Escherichia coli O157:H7 has emerged as an important gastrointestinal pathogen of human which cause several acute disease and mostly of them lead to death. milk are contaminated with different types of microorganisms at milk collection places milk is a major part of human food Baghdad city. Therefore rapid and sensitive detection of the bacterium with high specific plays a critical role in the control of the pathogenic bacteria. The aim of this study was based on Polymerase chain reaction (PCR) technique that amplified the uidR and flicH7 genes.