Detection of GM soybean in some processed food products in Russia

DETECTION OF GENETICALLY MODIFIED SOYBEAN IN SOME PROCESSED FOOD PRODUCTS IN RUSSIA This study aimed to compare two methods of DNA extraction. Twenty three samples of food products were used during DNA extraction. Laboratory including PCR machine Multichannel cycler “Tertsik-терцик” spectrophotometer horizontal gel electrophoresis apparatus & power supply UV transluminator and a number of reagents were used. From our results we noticed that the sodium dodecyl sulfate-SDS based method was better than Cetyl trimethyl ammonium bromide-CTAB based method. On the other hand among the samples used the presence of transgenes related to herbicide tolerant neomycin/kanamycin antibiotic resistance enzyme 5-enolpyruvyl-shikimate-3-phosphate synthase-CP4 EPSPS genes and 35s promoter from different species and nos terminator were detected.