DNA extraction from slug epithelial tissue

The aim of the research was to compare three protocols: Extraction with SDS; Extraction with CTAB and 2β-mercaptoethanol and Extraction with SDS and proteinase K to determine the most appropriate method to obtain DNA of high molecular weight and purity in slug epithelial tissue: Arion subfuscus and Deroceras reticulatum. DNA concentration and purity were estimated spectrophotometrically (absorbance at 260 nm and Abs260/Abs280 nm ratio). A completely randomized design with six replicates was used. Significant differences (p<0.01) were detected for amount concentration and purity between protocols. The protocol (SDS and proteinase K) allowed obtaining DNA with higher molecular weight (2 514.90±291.56) and purity (1.97±0.02). The quality of the DNA of each protocol was verified by PCR-RAPD observing little reproducibility for the OPA-02 primer (SDS protocol); while in the other methods high resolution amplification profiles and reproducible bands were observed. It is recommended to use the SDS protocol and proteinase K to obtain genomic DNA of high molecular weight quality and purity in slugs.