Effect of Synthetic Oxytocin on Neuroendocrine and Reproductive System

In mammals nonapeptide oxytocin (OT) exerts many different biological functions through G-protein-coupled transmembrane receptors-OTR and vasopressin receptors viz. V1 (V1a and V1b) and V2 type. OT is effective after administration by any parenteral route such as i.p. s.c. i.m. i.v. intranasal and orally. This thesis explored about the toxicity bioassay of synthetic OT on female mice. In connection to this 36 female mice were randomly allocated into 2 groups as control (n=18) and treated (n=18). The treated and control groups were received intraperitonial (i.p.) dose of synthetic OT (50mIU/g body weight) and physiological saline respectively for different duration i.e. 30 60 and 90 days from postnatal day 10±5. The body and relative organ weight; estrus cycle; hematological; serum biochemical and enzymological analysis; serum hormonal assay; biogenic amine estimations in total brain; and histopathological study were performed. Statistical analyses were performed by one way ANOVA and SEM. This research was done according to the protocol of Institutional Animals Ethics Committee (IAEC) Barkatullah University Bhopal India with Reg. No. 1885/GO/Re/S/16/CPCSEA/IAEC/BU/07.