Emergence of Ndm-1 Among Carbapenem Resistant Klebsiella Pneumoniae

Present study reports NDM-1 for the first time among Klebsiella pneumoniae from hospitalized patients in hospitals of Baghdad Iraq. Fifty-five clinical isolates of K. K. pneumoniae resistant to carbapenem were investigated from burned wounds sputum and blood samples. The susceptibility to different antibiotics was tested by the Vitek-2 system. All strains were multidrug resistant and they showed nine different antimicrobial resistant patterns (A- I). The phenotypic detection of carbapenemases by MASTDISCS D70C revealed 29(52.73%) strains were MBL producing phenotypically out of 55 carbapenem-resistant K. pneumoniae strains. The blaNDM-1 genes were detected by conventional PCR and the result showed 37(67.27%) strains positive for blaNDM-1 gene. Data obtained from blaNDM-1 gene sequencing was submitted to the GenBank under the accession numbers: KY524489 KY524488 KY497948 KY497947 KY501638 and KY501637.