Separation is a distinct area of physical and analytical chemistry. Professor J. Calvin Giddiogs from University of Utah first recognized this in 1960s and termed it as separation science. Separation is an operation by which a mixture is divided into fractionshaving different compositions. Separation is a process of isolation per-concentration and recovery of solute of interest from mixture of solutes.This study reveals that the batch extraction of ampicillin cephalexin cefedroxil land ciprofloxacin by ELM system (B) consisting cyanex -301- span 80-toluene is better than the ELM (A) system consisting Aliquat - 336 span - 80 - toluene the scale up study may therefore be confirmed using (B) system for real industrial samples.
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