EVALUATION OF ENDOPHYTIC FRACTIONS OF BOERHAAVIA DIFFUSA L. ROOTS

Endophytes are major originators of new bioactive compounds with fascinating pharmacological activities. Here we have isolated characterized and screened endophytic bacteria of Boerhaavia diffusa Linn (BDEF) root for antioxidant and hepatoprotective activities. BDEF was isolated from the roots and grown in nutrient agar media aseptically. The grown bacteria was further fermented in nutrient broth and extracted using chloroform (CBD) and ethyl acetate (EABD). CBD and EABD were assayed for antioxidant activities by different methods. The highest inhibition was exhibited in EABD with IC50 level of 22.86 μg/ml for DPPH and 82.78 μg/ml for hydroxyl radical. Further CBD and EABD were evaluated for antihepatotoxic activity against CCl4 induced hepatotoxicity. The results revealed that CBD and EABD at 200 mg/ kg p.o. restored the biochemical parameters against CCl4 induced hepatotoxicity to the normal values. BDEF was studied for rDNA sequencing by polymerase chain reaction technique. The endophytic bacterium was identified as Bacillus cereus based on its morphological and molecular characterization. CBD and EABD fractions have exhibited antioxidant and hepatoprotective activity.