FISH Technology

Fluorescence in situ hybridization (FISH) has been developed as a powerful technology which allows direct visualisation or localisation of genomic alterations. The technique has been adopted to a range of applications in both medicine especially in the areas of diagnostic cytogenetics and biology. Topics described in this manual include: FISH on native human tissues such as blood bone marrow epithelial cells hair root cells amniotic fluid cells human sperm cells; FISH on archival human tissues such as formalin fixed and paraffin embedded tissue sections cryofixed tissue; simultaneous detection of apoptosis and xpression of apoptosis-related genes; comparative genomic ybridization; and special FISH techniques.