Highly experienced researchers describe in step-by-step detail methods that have proven most useful in delivering genes to mammalian cells. Volume 1: Nonviral Gene Transfer Techniques focuses on gene delivery by a variety of chemical and physical methods including ultrasound biolistics peptides PNA clamps liposomes microinjection electroporation particle bombardment dendrimers and hydrodynamics. An accompanying volume Volume 2: Viral Gene Transfer Techniques details procedures for delivering genes to cells in vitro and in vivo including the use of lentiviral vectors.
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