Genetic profiling of CYP2D6 variants known to alter enzyme activity

CYP2D6 is responsible for the metabolism of 20‐25% of currently prescribed drugs and thus the study of its genetic variation are of particular importance to pharmacogenetics as the various combinations of variant alleles result in a diverse set of metabolic profiles. However little is currently known about the frequency of functionally important CYP2D6 SNPs in black South Africans. Therefore this study aims to genotype four functionally important SNPs across the coding region of CYP2D6 in order to determine the allelic frequencies in black South Africans. In this study 90 unrelated black Bantu‐speaking participants were genotyped using the tetra ARMS-PCR method for each of the four SNPs: rs16947 rs1135840 rs1065852 and rs28371706. These frequencies were compared with those from other populations using 1000 Genomes Project data. Knowledge of the novel frequencies of these polymorphisms and their resulting metabolic classes would prove important for reducing both adverse drug reactions and improving drug dosage calculations with respect to the black South African population.