This study investigates the genotoxic effects of paraquat a non-selective herbicide on maize (Zea mays) through controlled exposure at concentrations of 0.2 0.6 and 0.8 mg/L. Gel electrophoresis and clustering analysis were employed to assess DNA fragmentation and genetic response with distilled water-treated samples serving as controls. Results showed a concentration-dependent increase in DNA damage marked by intensified DNA band fragmentation at 0.6 and 0.8 mg/L concentrations indicating significant oxidative stress and potential genetic compromise. The control samples exhibited minimal genetic alteration highlighting the herbicide-free baseline. Clustering analysis supported these findings grouping higher concentrations together suggesting similar genotoxic impacts at near-toxicity thresholds. These results align with existing literature on herbicide-induced oxidative damage in maize and underscore the need for cautious paraquat application in agricultural contexts.
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