Identification of Bacillus producing thermotolerant hydrolases

Thermotolerant enzymes were produced from soil and water Bacillus species as protease (89.28%) (73.17%) amylase (55.35%) (51.21%) lipase (91.07%) (95.12%) cellulase (85.71%) (60.97%) and chitinase (85.71%) (92.68%) respectively after culturing on solid agar media with different incubation period at 50°C. So 41.23% of soil and water Bacillus isolates were detected for hydrolases production as (protease amylase lipase cellulase and chitinase) together and showed that B. polymyxa strains were the best bacteria in protease amylase and cellulase production whereas B. lentus strains was a good chitinase producer. Lipase was produced by several Bacillus strains as B. circulans B. stearothermophilus B. polymyxa B. mycoides and B. lentus after culturing on different media and incubation period at 50°C.