Impact of mononuclear cell cryopreservation

Develop the technique for separating CMNSP on Ficoll and thawing freezing then evaluate the impact of -80°C freezing in the short and long term on lymphocyte immunophenotyping data.Ten cases of SLPC referred to the CMF unit: 8 cases of SLPC-B (4 cases of CLL and 4 cases of SLPC-B other than CLL) and 1 case of SLPC-T (Sézary syndrome) and 1 case of SLPC with NK cells.Comparison of cell viability before freezing (D1) and after freezing at -80°C (D5 and D30) showed a significant decrease in cell viability after short-term (D5) and long-term (D30) freezing (p<10-3). Comparison of the percentages of expression of the Ac tested and the MFIs between D5 and D30 of freezing at -80°C in DMSO/SVF5% showed no statistically significant difference. Furthermore cryopreservation for 5 days and 30 days affected the Matutes score due to a change in the intensity of lambda light chain expression.This technique is simple to implement and could easily be included in research designs for the immunological phenotyping of SLPCs.