In Situ Hybridization in Light Microscopy

In Situ hybridization allows the visualization of specific DNA/RNA sequences in individual cells in tissue sections, single cells, or chromosome preparations, and is an especially important method for studying DNA and RNA in heterogeneous cell populations. This book delves into in situ hybridization methods through the use of light microscopy used by molecular biologists, pathologists, geneticists, and biochemists. It will also appeal to research scientists who are interested in visualizing methods for nucleic acids and proteins. The book features a two-column layout for protocols/principles, simplifies the techniques and offers many schemes and tables to help the reader choose the best procedure. Probes. Tissue Preparation. Pretreatments. Hybridization. Revelation of Hybrids. Counter-Staining and Modes of Observation. Controls and Problems. Typical Protocols. Examples of Observation. Appendices. Glossary. Index.