This book has prepared from an experimental result of sugarcane tissue culture it contains all paper preparation content. Tissue culture is a collection of techniques to grow plant cells tissues and organs under sterile conditions. It is also known as in vitro culture; an example is micro propagation in which a small piece of plant tissue is used to generate a whole plant. This technique is highly adopted in horticulture and forestry to allow rapid production of new superior variety of offspring besides land/space and time saving through direct and indirect methods using different parts of plants such as shoot tip leaf meristem buds seed etc.
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