In Vitro Toxicity of Cadmium Oxide Particles in BRL 3A Rat Liver Cells

<p>For the last decade or so in vitro toxicology has been studied as a method for determining human toxicity as well as hazard identification and characterization. One purpose of in vitro studies is to determine the possibility of developing systems in which the toxicity of chemicals can be quantified without heavy reliance on animal experimentation. Specific data pertaining to cadmium toxicity in humans through use of in vitro methods and models is sparse at best. There is a need to complete more studies and collect more data to study particular chemicals and their effects and to complete proper evaluation and comparison to in vitro studies on human cells. The need for such data is the overall reason for this thesis research and experimentation. Toxicology in vitro refers to a method of exposing cell tissue directly to the chemical compound under study. In this research rat liver cells or hepatocytes were cultured exposed to cadmium oxide particles and analyzed with an MTT assay. Experiments were carried out with doses ranging from 0.001 ??g/mL to 2.5 ??/mL for 6 12 and 24 hours. Two cell densities were also studied for each experiment 1000000 cells/mL and 500000 cells/mL in order to ascertain the effects of toxicity on cell density. The research led to the conclusion 1000000 cells/mL is more resistant to the toxicant than 500000 cells/mL and the toxicant is most toxic to the cells after 24 hours.</p><p>This work has been selected by scholars as being culturally important and is part of the knowledge base of civilization as we know it. This work was reproduced from the original artifact and remains as true to the original work as possible. Therefore you will see the original copyright references library stamps (as most of these works have been housed in our most important libraries around the world) and other notations in the work.</p><p>This work is in the public domain in the United States of America and possibly other nations. Within the United States you may freely copy and distribute this work as no entity (individual or corporate) has a copyright on the body of the work.</p><p>As a reproduction of a historical artifact this work may contain missing or blurred pages poor pictures errant marks etc. Scholars believe and we concur that this work is important enough to be preserved reproduced and made generally available to the public. We appreciate your support of the preservation process and thank you for being an important part of keeping this knowledge alive and relevant.</p>