Indirect Somatic Embryogenesis of Japonica Rice Through Anther Culture

Time utilization was observed in 16 growth regulator treatments of callus induction and 18 growth regulator treatments of regeneration. Data of callus induction and regeneration were analyzed with factorial completely randomized design (FCRD). The highest callus induction frequency was recorded in Azucena into callus induction treatment T4 containing 2 4-D 2 mg/l + Kinetin 1 mg/l (49.99%) followed by in Moroberekan into callus induction treatment T11 containing 2 4-D 1 mg/l + NAA 2 mg/l + Kinetin 0.5 mg/l (48.64%) in 17 weeks and 9 weeks. The highest shoot regeneration frequency was recorded in Azucena into shoot regeneration treatment T10 containing Kinetin 0.5 mg/l + BAP 2 mg/l + NAA 1 mg/l (83.33%) in 28 days followed by Moroberekan into shoot regeneration treatment T16 containing Kinetin 2 mg/l + BAP 1 mg/l + NAA 1 mg/l (80.0%) in 35 days. The highest plantlet regeneration frequency was recorded in Moroberekan into plantlet regeneration treatment T16 containing Kinetin 2 mg/l + BAP 1 mg/l + NAA 1 mg/l (80.0%) in 35 days followed by plantlet regeneration treatment T10 containing Kinetin 0.5 mg/l + BAP 2 mg/l + NAA 1 mg/l (55.55%) in 21 days.