Inhibition of Angiogenesis by Potential Chemopreventive Agents

A new human in vitro anti-angiogenesis assay was established and applied to identify novel angiopreventive agents. Subsequently two chemopreventive compounds xanthohumol (XN) from Humulus lupu­lus L. (hop) and sulforaphane (SFN) an isothiocyanate derived from cruciferous vegetables (Brassicaceae) were selected for detailed in­vestigation of their angiopreventive potential and mechanisms invol­ved in the inhibition of angiogenesis. Representative targets included effects at the transcriptional levels of pro-angiogenic factors anti-gelatinolytic and anti-endothelial activity. In vivo XN was shown to exhibit a strong anti-tumour potency on human breast cancer xeno­grafts mediated by the inhibition of tumour-induced angiogenesis. These findings strongly suggest that XN and SFN represent attractive chemopreventive lead structures possessing a remarkable potential to prevent tumour development.