Intracellular pH and its Measurement

This volume introduces a summary of all the techniques used to estimate pH reliably. Emphasis is placed on the techniques that provide the most reliable and detailed data. The role of cell pH is explained with special emphasis on enzymology and membrane transport and bioenergetics. This book was written especially for molecular biologists, biochemists and biophysicists. The Concept of pH, Hydrolysis, and Buffers. pH in Biological Systems. Methods of Determining Intracellular pH with Electrodes. Assays of Intracellular pH Using Chemical Probes: Principles of pH Indicator Response. Assays of Intracellular pH Using Chemical Probes: Distribution Techniques. Assays of Intracellular pH Using Chemical Probes: Absorption Spectroscopy. Assays of Intracellular pH Using Chemical Probes: Fluorescence Spectroscopy. Assays of Intracellular pH Using Chemical Probes: Nuclear Magnetic Resonance Spectroscopy. The Choice of the Assay Method. Heterogeneity of Intracellular pH. Index.