Introduction to Optical Microscopy

This fully updated self-contained textbook covering modern optical microscopy equips students with a solid understanding of the theory underlying a range of advanced techniques. Two new chapters cover pump-probe techniques and imaging in scattering media and additional material throughout covers light-sheet microscopy image scanning microscopy and much more. An array of practical techniques are discussed from classical phase contrast and confocal microscopy to holographic structured illumination multi-photon and coherent Raman microscopy and optical coherence tomography. Fundamental topics are also covered including Fourier optics partial coherence 3D imaging theory statistical optics and the physics of scattering and fluorescence. With a wealth of end-of-chapter problems and a solutions manual for instructors available online this is an invaluable book for electrical engineering biomedical engineering and physics students taking graduate courses on optical microscopy as well as advanced undergraduates professionals and researchers looking for an accessible introduction to the field.