Lysine production by Corynebacteruim glutamicum Homoserine (-)

The aim of our work is to study the effect of ammonium acetate as a carbon and nitrogen source on the productivity of homoserine (-) auxotrophic Corynebacterium glutamicum mutants. To this end we inoculated four media containing a homoserine (-) auxotrophic C.glutamicum mutant as a carbon source (glucose; glucose and ammonium acetate; ammonium acetate; ammonium acetate and sodium acetate). During this fermentation we monitored biomass production (by turbidimetry) and lysine production by the strain while measuring glucose and ammonium acetate consumption. We found that in an ammonium acetate-based medium fermentation was characterized by remarkable lysine production (11.45 g/l) and low biomass production.Key words: Corynebacterium glutamicum auxotrophs homoserine(-) mutation ammonium acetate glucose lysine feedback inhibition.