Method Development and Validation for Voriconazole Estimation

A rapid sensitive and specific RP-HPLC method with UV detection was developed and validated for the determination of voriconazole. Chromatography was performed on an Enable C18 G column (250 × 4.6 mm) in isocratic mode using a mobile phase of methanol and acetonitrile (60:40 v/v). The flow rate was set at 1 mL/min and the effluent was monitored at 223 nm. Chromatographic analysis revealed a main peak for voriconazole at a retention time of 2.841 minutes. Methanol was selected as the solvent for the analysis with the ?max determined to be 223 nm. The method exhibited linearity over a concentration range of 5-30 µg/mL. Validation parameters including linearity range precision accuracy limit of detection (LOD) and limit of quantitation (LOQ) were assessed and met the required standards.