Microbe supported enhanced production of Rosmarinic acid of medicinal plants in vitro

Scientific Study from the year 2017 in the subject Biology - Micro- and Molecular Biology language: English abstract: In the described study the bio-enhancement of Rosmarinic acid( RA) was done successfully in suspension culture of Salvia officinalis and Ocimum sanctum suspended with E coli K12 entrapped alginate beads.It is reported that Tyrosin Amino transferase has a key role in the production of Rosmarinic acid which is present in E coli K 12. Quantification by HPTLC indicated that the maximum RA content was noted in 4 weeks old (2050 mg/l) in Salvia officinalis suspension culture fed with Tyrosin 10 mg/100ml followed by 4 weeks old Ocimum sanctum suspension culture fed with Tyrosin 10 mg/100ml(715 mg/l) that are about 18 fold and 5 folds enhancement as compared to control. The method for significantly enhancement of RA was reported for the first time.