Molecular and Cell Biology

Globally breast cancer is ubiquitous and regarded as a leading cause of death amongst other causes of death. It plays a role in several aberrant genes seen in the tumorigenesis pathway thus making it heterogenic. An example of this gene is the HDAC1 gene which is a multifunctional protein and plays a role in gene expression regulation. Using the CRISPR/Cas9 system a remarkable gene editing approach essential in identifying and validating genomic targets with the potential to be tumoricidal the role of HDAC1 was investigated in a breast cancer cell line MCF-7.