Molecular cloning and characterization of recombinant human irisin

The extracellular domain of FNDC5 is proposed to be cleaved into a peptide hormone named ‘irisin’ after the Greek messenger goddess ‘Iris’. Irisin has been shown to play an important role in various physiological conditions. Irisin has been shown to be in increased levels in muscle tissue after exercise in some studies whereas others showed no effect of exercise on irisin levels. Several studies shown that irisin reduce insulin resistance adiposity and the effects of other adiposity-based pathological conditions. In this book we presented whether the commercial antibodies generated against various regions of the irisin/FNDC5 are capable of identify the full-length and truncated forms of irisin and reported the biological effects of recombinant irisin which were expressed in E. coli.