Molecular variation analysis in Date palm (Phoenix dactylifera)

In Date palm (Phoenix dactyliferaL.) molecular characterization to find the best genotype and male/female identification at an early stage before flowering is of great economic significance for farmers. Genetic finger printing using molecular markers is an important tool. In present investigation two types of marker viz. RAPD and ISSR were applied in 8 Date palm genotypes and five types of markers RAPD ISSR SSR ITS and 18s rRNA gene were applied in 5 genotypes of Date palm (Ghanshyam Early maturing Late maturing Male 2 small and Male1 tall) and at the same time Ghanshyam was also screened with its 14 seedling genotypesRAPD markers were more efficient than ISSR assay with regard to polymorphic detection; RAPD markers detected 36.84% polymorphism as compared to 0.12% by ISSR markers. Cluster analysis by UPGMA shows that the dendrogram obtained was similar for RAPD and RAPD + ISSR In other case study of 5 Date palm genotypes (Ghanshyam Early maturing Late maturing Male 2 small and Male1 tall) five type of markers with one best primer for each marker (RAPD ISSR SSR ITS and18s rRNA) were used.