Multi-Parametric Live Cell Microscopy of 3D Tissue Models

<p>This book provides an essential overview of existing state-of-the-art quantitative imaging methodologies and protocols (intensity-based ratiometric and FLIM/ PLIM). A variety of applications are covered including multi-parametric quantitative imaging in intestinal organoid culture autofluorescence imaging in cancer and stem cell biology Ca2+ imaging in neural <i>ex vivo</i> tissue models as well as multi-parametric imaging of pH and viscosity in cancer biology. The current state-of-the-art of 3D tissue models and their compatibility with live cell imaging is also covered. This is an ideal book for specialists working in tissue engineering and designing novel biomaterial.</p>