Murine Interferon Regulatory Factor-2 (IRF-2) and Escherichia coli

Interferon Regulatory Factor-2 is a vertebrate transcription factor having oncogenic nature; it antagonises Interferon Regulatory Factor-1 a tumor suppressor gene functions. Knockout mice for IRF-2 gene showed defect in B-cell lymphopoeisis they died following LCMV (Lymphocytic Choriomeningitis Virus) infections. IRF-2 is functionally just opposite of IRF-1. Interestingly one would have thought that as IRF-1-/- (knock-out) mice showed lack of CD8+T cells and low cytotoxicity of NK cells the over production of IRF-2 in mice may restore it. But it does not happen so the reason being unknown. However IRF-2 binds at Interferon Stimulated Response Element sequence in viral infected cell. This book is about mouse IRF-2 gene cDNA cloning and expression of chimeric protein in E.coli. This study will serve as a platform for further functional analysis of chimeric IRF-2 which is potent oncogene in aberrant conditions.