Non-viral Gene Therapy

Several years ago when the discovery of catalytic RNA was recognized in a public mannermany people asked if new ?elds of therapy would soon be available. Although some tentative positive answers were givennobody would say with certainty that RNA of various kinds was a truly promising means of altering gene expression. In factover the past decadeboth our knowledge of RNAs with different functions and the utility of RNA in the inhibition or enhancement of gene expression have occurred with great drama. We proceeded in terms of possible therapeutic tools from RNase P and group I introns through hammerhead RNA enzymes antisense technology and more recently to RNAi and its derivatives. A useful practical method of RNA delivery in animals will complete the picture. The diversity of RNA and the varied role of it inside cells and in therapy should be a tremendous challenge for young molecular biologists. This volume will make their task easier. Sidney Altman Sterling Professor of MolecularCellular & Devel- mental BiologyNobel Laureate Department of MolecularCellular and Developm- tal Biology Yale University V NGTPR 4/23/05 1:00 PM Page VI VI Foreword Delivery of nucleic acids to cells in an animal remains a challenging problem. It is the major obstacle to success of therapeutic approaches using genes and oli- nucleotidesincluding siRNAs. Solutions found so far by chemists are satisfactory only for transfection of cells in culture.