Phylogenetic Analysis of Viral Quasispecies

A new approach in the analysis of viral evolution is presented in this book an approach that merges Phylogenetic analysis and Bioinformatic techniques. Patterns of viral evolution are inferred from serially-sampled sequence data i.e. sequence data obtained from strains isolated at consecutive time points from a single patient or host. Traditional phylogenetic methods assume a tree-like evolutionary model while many RNA viruses have the capacity to exchange genetic material with one another using a process called re­combination. A genealogy involving recombination is best described by a net­work structure. A more general approach was implemented in a new compu­tational tool Sliding MinPD one that is mindful of the sampling times of the input sequences and that reconstructs the viral evolutionary relationships in the form of a network structure with implicit representations of recombi­nation events. The underlying network organization reveals unique patterns of viral evolution and could help explain the emergence of disease-associated mutants and drug-resistant strains with implications for patient prognosis and treatment strategies.