Holoptelea integrifolia a roadside plant are indicated by Charaka Samhitha Sushrutha Samhita and other traditional systems for the treatment of inflammation acid gastritis dyspepsia flatulence colic intestinal worms vomiting wounds vitiligo leprosy filariasis diabetes haemorrhoids dysmenorrhoea and rheumatism. The present study was aimed at macroscopic microscopic and chromatographic evaluation of leaves. The macroscopic microscopic evaluation is carried out in terms of organoleptic microscopic and physical parameter like fluorescence analysis treatment of plant drug with different chemical reagents .The dried leaves were subjected to successive Soxhlet extraction using petroleum ether chloroform ethyl acetate and methanol. Phytochemical evaluations to detect the different chemical principle present i.e. carbohydrates protein amino acids steroids glycocides alkaloids tannins and phenolic compounds. And to carry out chromatographic evaluation to isolate the chemical compounds. maroscopic microscopic and physiochemical determination and chromatography screening can be used as a diagnostic tool in the correct identification of the plants.
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