Production and Characterization of Bacterial Thermostable Cellulase

Project Report from the year 2018 in the subject Biology - Micro- and Molecular Biology grade: 0 language: English abstract: Cellulose is one the most abundant carbon sources present on the earth. In the book the author reported on production optimization and characterization of cellulolytic enzymes by thermophilic bacteria. Various adaptation strategies adapted by thermophilic bacteria are also discussed along with. The thermophilic bacterial strain produced cellulase in the CMC broth pH 7 containing 0.5% peptone 0.5% malt extract 0.2% ammonium sulphate 0.2% ammonium nitrate and 0.2% NaCl at 50?C. Interestingly the bacteria could not grow at 37?C confirming its thermophilic nature. Further the cellulase was characterized after getting partially purified by ammonium sulphate precipitation method. The partially purified cellulase may be employed to hydrolyze agricultural wastes to produce bioethanol i.e. biofuels. Thus the present research may help solve issues of crisis of renewable energy as well as environmental pollution.