Pulsed-Field Gel Electrophoresis

Burmeister and Ulanovskys new volume on Pulsed-Field Gel Electrophoresis presents step-by-step protocols for all users of this powerful methodology whether novice or expert. The book features a wide range of PFGE techniques auxiliary methods and a diverse array of powerful applications. Each protocol is given thorough treatment by authors who have had extensive hands-on-experience with it. Indeed many of the contributors have long provided assistance and troubleshooting advice to new users of PFGE during the early stages of its growth. Here they incorporate the benefits of their experience in the extensive Notes sections which describe common problems and pitfalls and offer sensible solutions for dealing with them. Topics and techniques treated include: FIGE CHEF OFAGE TAFE FORE ODPFGE preparation of DNA suitable for PFGE from: bacteria protozoa plants mammals and yeasts. preparation and analysis of yeast artificial chromosomes (YACS) mapping strategies biological applications theories and observations. In keeping with the well-known practicality of Humanas Methods in Molecular Biology series Pulsed-Field Gel Electrophoresis brims with protocols designed to work even the first time they are used.