Purification of Glossina Trypanolysin

WHO estimates there are 55 million people at risk of sleeping sickness transmitted by tsetse and 300000 of human cases of the disease occur each year with approximately 50 million cattle being at risk. Therefore there is a need for proper tsetse and trypanosomiasis control measures. The benefits of tsetse and trypanosomiasis control are enormous when viewed not ony with the narrow context of manpower and animal production but also within the border perspective that much of the tsetse infested land in the humid and sub-humid zones that is highly populated and has a relatively high agricultural potential particularly with respect to growing stable foods. So far the various conventional methods of control of trypanosomiasis and tsetse has proved to be difficult costly bleak and unsuccessful. Consequently it is very important to develop alternative control methods that are environmently safe cheep easily applied and affordable. Therefore midgut trypanolysin from Glossina morsitans morsitans was purified and characterized. Thus trypanolysin transgenesis by RLOs-the tsetse harbour in their midgut could be efficient way of interrupting trypanosomiasis cycle within the tsetse.