Quantitative Biomedical Optics

This is the textbook and reference resource that instructors students and researchers in biomedical optics have been waiting for. Comprehensive and up to date it covers a broad range of areas in biomedical optics from light interactions at the single-photon and single-biomolecule levels to the diffusion regime of light propagation in tissue. Subjects covered include spectroscopic techniques (fluorescence Raman infrared near-infrared and elastic scattering) imaging techniques (diffuse optical tomography photoacoustic imaging several forms of modern microscopy and optical coherence tomography) and laser-tissue interactions including optical tweezers. Topics are developed from the fundamental principles of physical science with intuitive explanations while rigorous mathematical formalisms of theoretical treatments are also provided. For each technique descriptions of relevant instrumentation and examples of biomedical applications are outlined and each chapter benefits from references and suggested resources for further reading and exercise problems with answers to selected problems.