<p><span style=color: rgba(23 43 77 1)>Ribosome biogenesis is a key cellular process involving the assembly of ribosomal proteins with ribosomal RNA (rRNA) into the complex three-dimensional structure of mature ribosomes. This Special Issue features cutting-edge original research articles as well as comprehensive reviews covering various aspects of ribosomal protein involvement in ribosome assembly with a focus on the following areas:· Methods to investigate the role of ribosomal proteins in ribosome biogenesis;· The regulation of ribosomal protein synthesis;· The nuclear import of ribosomal proteins and ribosomal protein chaperones;· The functions of ribosomal proteins in rRNA folding;· The order and mechanisms of incorporation of ribosomal proteins into nascent ribosomes;· The importance of correct ribosome assembly for the function of the mature ribosome.</span></p>