The anisotropic silver nano-particles were synthesized by using a simple efficient and eco-friendly method in the presence of Taxus baccata extract as reducing and stabilizing agent. The colloidal monodispersed nanoparticles were characterized by different methods including UV-Vis spectroscopy SEM TEM AFM EDS FTIR TGA DLS and Zetasizer. Following the investigation of anticancer activity of the nanoparticles on MCF-7 Caov-4 and HeLa cancer cell lines using MTT assay its mechanism was further investigated using flow cytometry and real-time PCR.