Simultaneous Estimation of Cefixime and Linezolid

Development and validation of simple precise accurate economical and sensitive HPLC assay method for the estimation of Cefixime and Linezolid in tablet dosage form. A chromatographic separation was achieved with a Hypersil BDS C18 (250mm4.6mm 5µm) analytical column. A Mixture of Phosphate Buffer (3.0 pH): methanol (40:60 v/v) was used as the mobile phase at a flow rate of 1.0 ml/min and detector wavelength at 277 nm. The retention time of Cefixime and linezolid was found to be 3.76 and 6.55 min respectively. Theoretical plates was 6896 and 4626 respectively and Tailing factor was 1.32 and 1.28. The linear ranges was 5-15 for cefixime and 15-45 ?g/ml for linezolid and the correlation coefficient was 0.999 for both drugs. The relative standard deviation was found to be less than 2%. A simple precise accurate economical and sensitive HPLC assay method have been developed and validated for the estimation of Cefixime and linezolid in tablet dosage form. All method validation parameters lie within its acceptance crieteria as per ICH Q2(R1) guideline.