<p>This book constitutes the refereed proceedings of the 31st International Symposium on String Processing and Information Retrieval SPIRE 2024 held in Puerto Vallarta Mexico during September 23���25 2024.</p><p>The 22 full papers and 4 short papers presented in this volume were carefully reviewed and selected from 41 submissions. The papers reflect the continuation of the long and well-established tradition of encouraging high-quality research at the broad nexus of string processing information retrieval and computational biology.</p>
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